Part:BBa_K2623021:Design

Bacterial outer membrane protein A (OmpA) fused with SpyTag at its N-termini and GFP at its C-termin

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 65
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1302
  

Design Notes

SpyTag is inserted to the C-termini or N-termini of the protein.

Source

partly from E.coli genomic sequence